CXCR2 Gα15 CHO
CBP71658
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| I. Background | |
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趋化因子是一类炎性细胞因子，具有调节细胞迁移、粘附、吞噬作用、细胞因子分 泌、增殖和细胞凋亡的能力。越来越多的证据表明，趋化因子及其受体在稳态和炎症条件 下在调节树突状细胞迁移以及稳态和炎症功能方面发挥着重要作用。 |
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| II. Description | |
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CXCR2 Gα15 CHO 药靶模型很好的模拟了体内 CXCR2的信号转导过程，原理见下图所示。
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| III. Introduction | |
| Host Cell: |
CHO |
| Expressed gene: | CXCR2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K + 10% FBS + 5 μg/ml Puromycin + 800μg/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CXCR2 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
Figure 2. Recombinant CXCR2 Gα15 CHO stably expressing CXCR2.
Figure 3. HTRF IP-One Assay with CXCR2 Gα15 CHO( C23).
Figure 4. HTRF cAMP Assay with CXCR2 Gα15 CHO( C23).
