GPR3 β-Arrestin CHO
CBP71676
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| I. Background | |
| GPR3 是一种组成性激活的孤儿 G 蛋白偶联受体，属于 A 类视紫红质样受体家族。它无需配体结合即可自发激活 Gs 蛋白，持续升高细胞内 cAMP 水平，在维持卵母细胞减数分裂停滞、神经元功能以及阿尔茨海默病相关的 β-淀粉样蛋白生成中发挥关键作用。近年研究还提示，油酰乙醇酰胺（OEA）可能是其内源性激动剂。 | |
| II. Description | |
| GPR3 β-Arrestin CHO 报告基因药靶模型很好的模拟了体内 GPR3/β-Arrestin 的信号转导过程。 |
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| III. Introduction | |
| Expressed gene: |
GPR3 |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+ 10% FBS +5 μg/ml Puromycin + 5 μg/ml Blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GPR3 Receptor |
| Assay Format: | β-Arrestin |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
V. Representative Data Figure 1. Dose Response of Inverse Agonists in GPR3 β-Arrestin CHO (C41). |
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